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KMID : 0903619930340020108
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Journal of the Korean Society for Horticultural Science
1993 Volume.34 No. 2 p.108 ~ p.114
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Development of Mass Production System by Somatic Embryogenesis in Oenanthe Stolonifera DC .
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Abstract
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This study was carried out to examine the appropriate explant sources and suitable growth regulators for somatic embryogenesis to establish a rapid mass production system in Oenanthe stolonifera DC. Immature florets were found to be the most competent explant sources for embryogenic callus formation. 2,4-D at 1.0 ㎎/ℓ was highly effective for the formation of embryogenic callus but inhibitory for the development and differentiation of somatic embryo. Somatic embryos were developed from the translucent and friable embryogenic callus with ivory color. Morphological characteristics of somatic embryogenesis were typical. Addition of BA promoted the callus growth synergistically with NAA and 2,4-D, but the production of embryogenic callus was inhibited by BA. Liquid media were more effective for the production of somatic embryos than solidified media.
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